Chlamydomonas reinhardtii snapgene files

For example, waveform analysis of live mutant cells shows that outer arm dynein is important for flagella to beat at high frequency, whereas the inner arm dyneins are important to produce a proper waveform ( Brokaw and Kamiya, 1987).

By quantifying the motility in wild type and mutants, we can infer the function of particular axonemal structure or proteins. Also important is the fact that we can relatively easily observe their flagellar motility in live cells, in demembranated and reactivated cells (вЂњcell modelsвЂќ), and in isolated axonemes. This is largely because this organism offers a variety of motility-deficient mutants, and we can analyze the defects with biochemical and molecular biological techniques. Ritsu Kamiya, in Methods in Cell Biology, 2009 I IntroductionД¬hlamydomonas reinhardtii has thus far provided the most detailed information about axonemal structure and function. The growth of the cells can easily be synchronized by lightвЂ“dark cycles. reinhardtii cells grown in the dark maintain a functional photosynthetic apparatus because of their ability to synthesize chlorophyll in the absence of light. Cells can thus be grown under three different conditions: in minimal medium with light and CO 2 as the sole carbon source (phototrophic growth), in acetate-containing medium with light (mixotrophic growth) or without light (heterotrophic growth). This feature has been exploited extensively for isolating and maintaining mutants deficient in photosynthetic activity. In the presence of acetate in the growth medium, the photosynthetic function of C. Weinjeim: VHC Verlagsgesellschaft, with permission from VHC Verlagsgesellschaft. Encyclopedia of Molecular Biology and Molecular Medicine, vol. Reproduced from Rocheix J-D (1996) Chalmydomonas. Section through a cell of Chlamydomonas reinhardtii, showing cup-shaped chloroplast (c) with thylakoid membranes, prominent nucleus (n), and mitochondria (m).

Chlamydomonas reinhardtii snapgene files